lytD [2019-07-09 11:39:33]

peptidoglycan N-acetylglucosaminidase

Locus: BSU35780

Isoelectric point: 9.94

Molecular weight: 95.36 kDa

Protein length: 880 aa

Gene length: 2643 bp

Function: major autolysin, cell separation

Product: peptidoglycan N-acetylglucosaminidase

Essential: no

E.C.: 3.2.1.96

Synonyms: cwlG

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → N-acetyl-β-D-glucosaminidases
• Groups of genes → Secreted proteins

Gene

Coordinates: 3,684,826 3,687,468

The protein

Catalyzed reaction/ biological activity

• hydrolysis of the glycosidic bond between N-acetyl--d-glucosamine residues and adjacent monosaccharides PubMed

Protein family

• glycosyl hydrolase 73 family (according to Swiss-Prot)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: lytD
  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

Biological materials

Mutant

• 1A788 ( lytD::tet), PubMed, available at BGSC
• 1A792 ( lytD::tet), PubMed, available at BGSC
• BKE35780 (lytD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT, downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC
• BKK35780 (lytD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT, downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC

References

Reviews

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Original publications

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